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Published-Ahead-of-Print April 4, 2007, DOI:10.2164/jandrol.106.002360

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Filling time of a lamellar capillary-filling semen analysis chamber is a rapid, precise, and accurate method to assess viscosity of seminal plasma

Sanne Rijnders , Jan G.M. Bolscher , Joseph McDonnell , and Jan P. Vermeiden *

* To whom correspondence should be addressed. E-mail: j.vermeiden{at}xs4all.nl.

In this study we demonstrate that capillary loaded disposable chambers for semen analysis can be used to assess the viscosity of seminal plasma in an accurate and precise manner. Using a viscosity meter the viscosity of seminal plasma samples was measured. We also assessed the filling of these samples in two types of capillary loaded chambers (2 and 4 chamber, capillary length 21 mm, 20 micron chamber depth). A linear relation between filling time and viscosity for viscosities up to of 8.4 cP was found. The accuracy of both methods ( viscosity meter and capillary filled chambers) was assessed with water and culture medium. The precision was assessed with repeated measurements of pooled filtered samples of seminal plasma. The coefficient of variation of both methods was 2-5%. The viscosity of 89% of the samples could be assessed with this method, 11 % were too viscous. The median value viscosity of these 89% was 4.1 cP and 95% of the viscosities were between 2.8 and 6.8 cP. It is concluded that the filling time of capillary loaded chambers can be used as a rapid and convenient method for accurate and precise assessment of the viscosity of seminal plasma.


Key words: Fertility • Semen • Semen Analysis • capillary filled slide • viscosity






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