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Published-Ahead-of-Print November 29, 2006, DOI:10.2164/jandrol.106.001552

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Standardization and Quality Control for Determination of Fructose in Seminal Plasma

Jin-Chun Lu *, Fang Chen , Hui-Ru Xu , Yu-Feng Huang , and Nian-Qing Lu

* To whom correspondence should be addressed. E-mail: jclu{at}jlonline.com.

Background: Few reports exist on standardization and quality control for the biochemistry analysis of seminal plasma. Methods: Fructose and sperm concentrations of each sample were assayed by the resorcinol method and computer-assisted sperm analysis system, respectively. Fructose concentrations in seminal plasma with or without chymotrypin, and obtained by centrifugation at different velocity, were also assayed. Two samples of seminal plasma, centrifuged at 3000 g for 15 min, were frozen for 20 days, and each was determined for their fructose concentrations after thawing every other day. Results: Standard fructose solution was relatively stable 2 weeks after preparation. There was no significant difference of fructose concentrations in seminal plasma among different standing durations (P>0.05). However, fructose concentration decreased with length of standing duration of semen, and there was a significant positive correlation between the decrease of fructose concentration and motile sperm concentration. There was a significant difference of sperm concentrations in seminal plasma obtained by centrifugation at different velocity, but there was no obvious difference of fructose concentration. Both chymotrypin and freezing-thawing had no apparent effect on determination of fructose in seminal plasma. Conclusions: Our results indicated that standard fructose solution, standing time of semen and centrifugation velocity should all be standardized, and frozen seminal plasma could serve as quality control products among different laboratories.


Key words: centrifugation velocity • freezing-thawing • fructose • quality control • standardization






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