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Published-Ahead-of-Print June 14, 2006, DOI:10.2164/jandrol.106.000950

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The Sycp-1Cre Transgenic Mouse and Male Germ Cell Inhibition of NF-{kappa}B

Reza John Rasoulpour and Kim Boekelheide *

* To whom correspondence should be addressed. E-mail: kim_boekelheide{at}brown.edu.

Successful spermatogenesis requires autocrine, paracrine and endocrine signaling throughout the testis. The seminiferous tubules contain somatic Sertoli cells in tight association with numerous germ cell populations. To address the in vivo biological roles of genes during spermatogenesis, spatial and temporal restriction of gene inhibition is a useful approach. To this end, Cre-LoxP technology can produce cell-specific knockdowns of genes, allowing dissection of the underlying processes that manifest as functional deficits in whole animals. Here we report the use of the synaptonemal complex protein 1-Cre (Sycp1-Cre) to create germ cell specific NF-{kappa}B knockdown mice through floxed IKK{beta} (Ikk{beta}F/F). We observed a ~43% LoxP gene recombination rate using the Sycp1-Cre, as determined by offspring genotype. In addition, we confirm that, with multiple generations, the LoxP sites fail to recombine due to epigenetic modification. This detailed examination of the meiotic Sycp1-Cre recombinase activity highlights the obstacles to germ cell specific gene inhibition through Cre/LoxP technology in the testis. Taken together, these data demonstrate a need for early spermatogonial expression of Cre recombinase, as an alternative to meiotic Cre expression, for the creation of germ cell-specific knockout mice.


Key words: Spermatogenesis • Testis




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R. J. Rasoulpour and K. Boekelheide
NF-kappaB Activation Elicited by Ionizing Radiation Is Proapoptotic in Testis
Biol Reprod, February 1, 2007; 76(2): 279 - 285.
[Abstract] [Full Text] [PDF]


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