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Published-Ahead-of-Print August 23, 2006, DOI:10.2164/jandrol.106.000182

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Calcium-modulated Rod Outer Segment Membrane Guanylate Cyclase type 1 Transduction Machinery in the Testes

Anna Jankowska *, Beata Burczynska , Teresa Duda , Jerzy B. Warchol , and Rameshwar K. Sharma

* To whom correspondence should be addressed. E-mail: ajanko{at}amp.edu.pl.

The importance of the second messengers, Ca2+ and cyclic GMP, for the process of fertilization is well established, the mechanisms for their intracellular regulations in the testes are, however, poorly understood. This study documents the biochemical, molecular, and functional identity of a Ca2+-modulated membrane guanylate cyclase transduction machinery in bovine testes. The machinery is both inhibited and stimulated by free Ca2+ levels. The Ca2+-sensor component of the inhibitory mode of the machinery is GCAP1 (guanylate cyclase activating protein type 1) and for the stimulatory mode is S100B. The transduction component is a Ca2+-driven rod outer segment membrane guanylate cyclase type 1, ROS-GC1. The cyclase is predominantly expressed in spermatogenic cells. GCAP1 expression is restricted to a small population of spermatogonia whereas S100B is present in the majority of spermatocytes and spermatids. The expression of GCAP1 and S100B in spermatocytes and spermatids is mutually exclusive.mode of the machinery is GCAP1 and for the stimulatory mode is S100B. The transduction component is the guanylate cyclase ROS-GC1. This is the first report on the existence of Ca2+-modulated ROS-GC transduction machinery in the testes.


Key words: Reproductive Tract • Calcium • GCAP1 • Membrane guanylate cyclase • ROS-GC1 • Signal transduction






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