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Published-Ahead-of-Print April 1, 2006, DOI:10.2164/jandrol.05142

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Appearance of Hyaluronan Binding Protein 1 Proprotein in Pachytene Spermatocytes and Round Spermatids Correlates with Spermatogenesis

Sonu Chand Thakur , Vinod Kumar , Ilora Ghosh , Archana Bharadwaj , and Kasturi Datta

The proprotein form of hyaluronan binding protein 1 (HABP1) has been reported to be present specifically in the pachytene spermatocytes and the round spermatids of the adult testis (Bharadwaj et al., 2002: Mol Reprod Dev 622: 223-232). In order to explore the role of HABP1 proprotein in spermatogenesis, its expression in adult rat testis was compared with that in developing rat testis, and in testis from estradiol treated adult rats (where spermatogenesis is arrested). Immunoblotting revealed that the mature form of HABP1 was consistently present in the testis, but its precursor form was not found in the testis of 7,14,21 and 28 day old animals. However, immunohistochemical analysis demonstrated the presence of the proprotein form in the pachytene spermatocytes and the round spermatids of the 21 and the 28 day old testis and this appearance correlated well with the appearance of these cells during spermatogenesis. RT-PCR analysis revealed transcriptional upregulation of HABP1 in adult testis when compared to the developing rat testis. Finally, loss of HABP1 proprotein expression from the pachytene spermatocytes and round spermatids was observed in the spermatogenic arrested adult rat testis. These results, collectively, demonstrate the appearance of HABP1 proprotein in the pachytene spermatocytes and the round spermatids during the initial stages of postnatal testis development and also suggest that this expression may be crucial for spermatogenesis.






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