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Published-Ahead-of-Print January 24, 2007, DOI:10.2164/jandrol.106.000869
Journal of Andrology, Vol. 28, No. 4, July/August 2007
Copyright © American Society of Andrology
DOI: 10.2164/jandrol.106.000869

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Damage to Chromosomes and DNA of Rhesus Monkey Sperm Following Cryopreservation

MING-WEN LI*, STUART MEYERS{dagger}, THEODORE L. TOLLNER* AND JAMES W. OVERSTREET*

From the * Division of Reproductive Biology, Department of Obstetrics and Gynecology, School of Medicine and {dagger} Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, Davis, California.

Correspondence to: Dr Stuart Meyers, One Shields Avenue, Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616 (e-mail: smeyers{at}ucdavis.edu).


Fresh and frozen-thawed rhesus monkey sperm were analyzed for DNA damage using the comet assay and for chromosome damage by cytogenetic analysis after intracytoplasmic sperm injection (ICSI) into mouse oocytes. The percentage of fresh sperm with damaged DNA in ejaculated semen was 0 to 2.7% (n = 5). Conventional cryopreservation and storage in liquid nitrogen caused DNA damage in 25.3% to 43.7% of sperm; when sperm were frozen without cryoprotectants, 52.7% to 92.0% of thawed sperm had DNA damage. However, no significant difference in chromosome damage was found between fresh sperm and frozen-thawed sperm when motile sperm were selected for ICSI. The percentage of sperm with abnormal karyotypes ranged from 0 to 8.3%. The most common structural chromosomal abnormalities in fresh motile sperm and frozen-thawed motile sperm were chromosome breaks or fragments. Our findings suggest that genetically competent frozen-thawed macaque sperm can be selected for fertilization by using only motile sperm for ICSI.

     Key words: Macaque, spermatozoa, ICSI




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Q. Dong, S. E. Rodenburg, C. Huang, and C. A. Vandevoort
Cryopreservation of Rhesus Monkey (Macaca mulatta) Epididymal Spermatozoa Before and After Refrigerated Storage
J Androl, May 1, 2008; 29(3): 283 - 292.
[Abstract] [Full Text] [PDF]


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Copyright © 2007 by The American Society of Andrology.