Journal of Andrology
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Journal of Andrology, Vol 8, Issue 6 398-402, Copyright © 1987 by The American Society of Andrology

JOURNAL ARTICLE

Uptake and release of ascorbic acid by rat Leydig cells in vitro

W. H. Moger
Department of Physiology, Dalhousie University, Halifax, Nova Scotia.

The mechanism by which ascorbic acid is transported into rat Leydig cells in vitro was investigated. Transport of [14C]ascorbic acid was temperature-dependent and inhibited in the absence of sodium in the incubation medium. The uptake of [14C]ascorbic acid was saturable (apparent Km approximately equal to 33 microM, Vmax approximately equal to 20 pmol/10(6) cells/min) and stereospecific. Uptake was not affected by removing glucose from the incubation medium, indicating that no significant uptake was occurring via the glucose transport system. Transport of [14C]ascorbic acid into Leydig cells was not affected by testosterone concentrations up to 7 microM or by LH at a concentration that causes maximum androgen secretion. Leydig cells preloaded with [14C]ascorbic acid rapidly released radioactivity into the incubation medium, a process that was unaffected by the presence of LH. The results suggest that ascorbic acid uptake by Leydig cells is by an electroneutral, sodium-dependent co-transport mechanism as in some, but not all, other types of cells.


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T. G. Zreik, P. H. Kodaman, E. E. Jones, D. L. Olive, and H. Behrman
Identification and characterization of an ascorbic acid transporter in human granulosa–lutein cells
Mol. Hum. Reprod., April 1, 1999; 5(4): 299 - 302.
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