| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Journal of Andrology, Vol 8, Issue 5 338-348, Copyright © 1987 by The American Society of Andrology
JOURNAL ARTICLE |
J. G. Alvarez, J. C. Touchstone, L. Blasco and B. T. Storey
Department of Obstetrics and Gynecology, University of Pennsylvania School of Medicine, Philadelphia 19104-6080.
Spontaneous lipid peroxidation in washed human spermatozoa was induced by aerobic incubation at 32 C and measured by malonaldehyde production; loss of motility during the incubation was determined simultaneously. Malonaldehyde production at the point of complete loss of motility, defined as the lipoperoxidative lethal endpoint (LLE), was 0.10 +/- 0.03 nmol/10(8) cells (mean +/- SD, n = 40), and was independent of the time to complete loss of motility. Human spermatozoa produced both H2O2 and O2-. during aerobic incubation. Inhibition of superoxide dismutase in these cells with KCN showed that all the H2O2 production is due to action of the dismutase. The superoxide dismutase activity of individual human sperm samples varied between 1 and 10 U/10(8) cells, variations between samples from a single donor being nearly as great as those between different donors. The time to complete motility loss (tL) showed equal variation of 1 to 10 hours among samples. The rate of spontaneous lipid peroxidation, calculated as LLE/tL, for a given sperm sample and the superoxide dismutase activity of the same sample, determined prior to aerobic incubation, gave a good linear correlation (r = 0.97). Glutathione reductase, glutathione peroxidase, and glutathione were found to be present in human spermatozoa, but showed little variation among samples. These results suggest that superoxide dismutase plays the major role in protecting human spermatozoa against lipid peroxidation. In addition, the superoxide dismutase activity of a fresh sperm sample appears to be a good predictor of the lifetime (up to the complete loss of motility) of that particular sample, and so may prove useful in semen analysis.
This article has been cited by other articles:
 |
|
 |
|
  E. Marti, J. I. Marti, T. Muino-Blanco, and J. A. Cebrian-Perez Effect of the Cryopreservation Process on the Activity and Immunolocalization of Antioxidant Enzymes in Ram Spermatozoa J Androl, July 1, 2008; 29(4): 459 - 467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.J. Aitken, J. K. Wingate, G. N. De Iuliis, and E. A. McLaughlin Analysis of lipid peroxidation in human spermatozoa using BODIPY C11 Mol. Hum. Reprod., April 1, 2007; 13(4): 203 - 211. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I.M.W. Ebisch, C.M.G. Thomas, W.H.M. Peters, D.D.M. Braat, and R.P.M. Steegers-Theunissen The importance of folate, zinc and antioxidants in the pathogenesis and prevention of subfertility Hum. Reprod. Update, March 1, 2007; 13(2): 163 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. P. Weir and B. Robaire Spermatozoa Have Decreased Antioxidant Enzymatic Capacity and Increased Reactive Oxygen Species Production During Aging in the Brown Norway Rat J Androl, March 1, 2007; 28(2): 229 - 240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sarica, M. Corduk, M. Suicmez, F. Cedden, M. Yildirim, and K. Kilinc The Effects of Dietary L-Carnitine Supplementation on Semen Traits, Reproductive Parameters, and Testicular Histology of Japanese Quail Breeders J. Appl. Poult. Res., January 1, 2007; 16(2): 178 - 186. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Nagdas, V. P. Winfrey, and G. E. Olson Identification of a Hamster Sperm 26-Kilodalton Dehydrogenase/Reductase That Is Exclusively Localized to the Mitochondria of the Flagellum Biol Reprod, August 1, 2006; 75(2): 197 - 202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. N. De Iuliis, J. K. Wingate, A. J. Koppers, E. A. McLaughlin, and R. J. Aitken Definitive Evidence for the Nonmitochondrial Production of Superoxide Anion by Human Spermatozoa J. Clin. Endocrinol. Metab., May 1, 2006; 91(5): 1968 - 1975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Georgiou, E. Sostaric, C. H. Wong, A. P. L. Snijders, P. C. Wright, H. D. Moore, and A. Fazeli Gametes Alter the Oviductal Secretory Proteome Mol. Cell. Proteomics, November 1, 2005; 4(11): 1785 - 1796. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Starr, C. Chen, D. R. Doody, L. Hsu, S. Ricks, N. S. Weiss, and S. M. Schwartz Risk of Testicular Germ Cell Cancer in Relation to Variation in Maternal and Offspring Cytochrome P450 Genes Involved in Catechol Estrogen Metabolism Cancer Epidemiol. Biomarkers Prev., September 1, 2005; 14(9): 2183 - 2190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Meseguer, N. Garrido, C. Simon, A. Pellicer, and J. Remohi Concentration of Glutathione and Expression of Glutathione Peroxidases 1 and 4 in Fresh Sperm Provide a Forecast of the Outcome of Cryopreservation of Human Spermatozoa J Androl, September 1, 2004; 25(5): 773 - 780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.C.L. Ford Regulation of sperm function by reactive oxygen species Hum. Reprod. Update, September 1, 2004; 10(5): 387 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Dobrzynska, A. Baumgartner, and D. Anderson Antioxidants modulate thyroid hormone- and noradrenaline-induced DNA damage in human sperm Mutagenesis, July 1, 2004; 19(4): 325 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Aitken, M. A. Baker, and M. O'Bryan Shedding Light on Chemiluminescence: The Application of Chemiluminescence in Diagnostic Andrology J Androl, July 1, 2004; 25(4): 455 - 465. [Full Text] [PDF]
|
|
|
|
|
|
S. B. DuTeaux, T. Berger, R. A. Hess, B. L. Sartini, and M. G. Miller Male Reproductive Toxicity of Trichloroethylene: Sperm Protein Oxidation and Decreased Fertilizing Ability Biol Reprod, May 1, 2004; 70(5): 1518 - 1526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Sikka Role of Oxidative Stress and Antioxidants in Andrology and Assisted Reproductive Technology J Androl, January 1, 2004; 25(1): 5 - 18. [Full Text] [PDF]
|
|
|
|
|
|
R.J. Aitken, A.L. Ryan, B.J. Curry, and M.A. Baker Multiple forms of redox activity in populations of human spermatozoa Mol. Hum. Reprod., November 1, 2003; 9(11): 645 - 661. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Alvarez Nurture vs Nature: How Can We Optimize Sperm Quality? J Androl, September 1, 2003; 24(5): 640 - 648. [Full Text] [PDF]
|
|
|
|
|
|
H. Chen, P. H. Chow, S. K. Cheng, A. L. M. Cheung, L. Y. L. Cheng, and W.-S. O Male Genital Tract Antioxidant Enzymes: Their Source, Function in the Female, and Ability to Preserve Sperm DNA Integrity in the Golden Hamster J Androl, September 1, 2003; 24(5): 704 - 711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Baumber, B. A. Ball, J. J. Linfor, and S. A. Meyers Reactive Oxygen Species and Cryopreservation Promote DNA Fragmentation in Equine Spermatozoa J Androl, July 1, 2003; 24(4): 621 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Schuffner, M. Morshedi, and S. Oehninger Cryopreservation of fractionated, highly motile human spermatozoa: effect on membrane phosphatidylserine externalization and lipid peroxidation Hum. Reprod., October 1, 2001; 16(10): 2148 - 2153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Vernet, N. Fulton, C. Wallace, and R. J. Aitken Analysis of Reactive Oxygen Species Generating Systems in Rat Epididymal Spermatozoa Biol Reprod, October 1, 2001; 65(4): 1102 - 1113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ollero, E. Gil-Guzman, M. C. Lopez, R. K. Sharma, A. Agarwal, K. Larson, D. Evenson, A. J. Thomas Jr, and J. G. Alvarez Characterization of subsets of human spermatozoa at different stages of maturation: implications in the diagnosis and treatment of male infertility Hum. Reprod., September 1, 2001; 16(9): 1912 - 1921. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Gil-Guzman, M. Ollero, M.C. Lopez, R.K. Sharma, J.G. Alvarez, A.J. Thomas Jr, and A. Agarwal Differential production of reactive oxygen species by subsets of human spermatozoa at different stages of maturation Hum. Reprod., September 1, 2001; 16(9): 1922 - 1930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Aguilar-Mahecha, B. F. Hales, and B. Robaire Expression of Stress Response Genes in Germ Cells During Spermatogenesis Biol Reprod, July 1, 2001; 65(1): 119 - 127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Saez, C. Motta, D. Boucher, and G. Grizard Prostasomes inhibit the NADPH oxidase activity of human neutrophils Mol. Hum. Reprod., October 1, 2000; 6(10): 883 - 891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Barroso, M. Morshedi, and S. Oehninger Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa Hum. Reprod., June 1, 2000; 15(6): 1338 - 1344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lenzi, L. Gandini, V. Maresca, R. Rago, P. Sgro, F. Dondero, and M. Picardo Fatty acid composition of spermatozoa and immature germ cells Mol. Hum. Reprod., March 1, 2000; 6(3): 226 - 231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Sharma, F. F. Pasqualotto, D. R. Nelson, A. J. Thomas Jr, and A. Agarwal The reactive oxygen speciestotal antioxidant capacity score is a new measure of oxidative stress to predict male infertility Hum. Reprod., November 1, 1999; 14(11): 2801 - 2807. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. El Mouatassim, P. Guerin, and Y. Menezo Expression of genes encoding antioxidant enzymes in human and mouse oocytes during the final stages of maturation Mol. Hum. Reprod., August 1, 1999; 5(8): 720 - 725. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. J. D'Cruz, Y. Dong, and F. M. Uckun Spermicidal Activity of Oxovanadium(IV) Complexes of 1,10-Phenanthroline, 2,2'-Bipyridyl, 5'-Bromo-2'-Hydroxyacetophenone and Derivatives in Humans Biol Reprod, February 1, 1999; 60(2): 435 - 444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Aitken, E. Gordon, D. Harkiss, J. P. Twigg, P. Milne, Z. Jennings, and D. S. Irvine Relative Impact of Oxidative Stress on the Functional Competence and Genomic Integrity of Human Spermatozoa Biol Reprod, November 1, 1998; 59(5): 1037 - 1046. [Abstract] [Full Text]
|
|
|
|
|
|
F. Tramer, F. Rocco, F. Micali, G. Sandri, and E. Panfili Antioxidant Systems in Rat Epididymal Spermatozoa Biol Reprod, October 1, 1998; 59(4): 753 - 758. [Abstract] [Full Text]
|
|
|
|
|
|
D. Mruk, C.-H. Cheng, Y.-H. Cheng, M.-y. Mo, J. Grima, B. Silvestrini, W. M. Lee, and C. Y. Cheng Rat Testicular Extracellular Superoxide Dismutase: Its Purification, Cellular Distribution, and Regulation Biol Reprod, August 1, 1998; 59(2): 298 - 308. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
