Journal of Andrology Cross-Journal Searching
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Usselman, M. C.
Right arrow Articles by Rossignol, D. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Usselman, M. C.
Right arrow Articles by Rossignol, D. P.

Journal of Andrology, Vol 6, Issue 5 315-320, Copyright © 1985 by The American Society of Andrology

JOURNAL ARTICLE

Rat cauda epididymal fluid is a mucus

M. C. Usselman, R. A. Cone and D. P. Rossignol

Immobilin, the highly viscoelastic glycoprotein isolated from rat cauda epididymal fluid, exhibits all of the key biochemical characteristics of a mucin: 1) it has a very high molecular weight (will not pass through a 10(6) dalton cut-off filter; 2) it contains 56% carbohydrate, with low or undetectable levels of mannose, xylose and uronic acid; 3) the carbohydrates (primarily galactose, N-acetylglucosamine and N-acetylgalactosamine) are arranged in short, oligosaccharide chains (4-20 monosaccharides per chain); 4) these oligosaccharide chains can be cleaved by NaOH in the presence of NaBH4, suggesting O-glycosidic linkages; and 5) the protein core is pronase-resistant. Immobilin, however, contains no detectable sialic acid, and 67% of the oligosaccharides are uncharged, indicating that immobilin is less acidic than most other mucins.


This article has been cited by other articles:


Home page
 Mol Hum ReprodHome page
G. Frenette, C. Legare, F. Saez, and R. Sullivan
Macrophage migration inhibitory factor in the human epididymis and semen
Mol. Hum. Reprod., August 1, 2005; 11(8): 575 - 582.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1985 by The American Society of Andrology.