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Journal of Andrology, Vol 4, Issue 4 248-252, Copyright © 1983 by The American Society of Andrology

JOURNAL ARTICLE

Evidence for the presence of androgen receptors in purified rat Leydig cells

S. Gulizia, R. D'Agata, B. M. Sanborn and E. Steinberger

Leydig cells, purified on two sequential Percoll gradients to purities of 89 +/- 1%, were used to study the binding of 17-beta-hydroxy-17 alpha-methyl-estra-4,9,11-triene-3-one(3H-R1881). The accumulation of 3H-R1881 in the nuclear fraction of these cells was time- and temperature-dependent. Specific binding was saturable with an apparent Ka of 0.14 nM-1 and a single class of binding sites at a concentration of 721 fmol/mg DNA. A fraction of the bound radioactivity in the nuclear pellet could be extracted with 0.4 M KCl, and a portion of this extracted steroid was associated with macromolecular species. The nuclear accumulation was androgen-specific. These data are consistent with the presence of androgen receptors in rat Leydig cells.


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[Abstract] [Full Text]


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Copyright © 1983 by The American Society of Andrology.