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1 University of Texas Medical
School at Houston, Department of
Obstetrics and Gynecology, Houston Texas
Parameters influencing the zona-free hamster egg
bioassay for human sperm fertility potential were evaluated. Egg recovery from hamsters superovulated at
random with respect to their estrous cycle was enhanced when the PMS-HCG time interval was
lengthened. Prolonged egg exposure to trypsin during
zona removal did not adversely affect egg viability. A
swim-up procedure for washing human sperm was superior to a centrifugation-resuspension technique, although the yield of washed cells was low. The kinetics
of sperm capacitation were examined for six different
donors over a 22-hour time period. Capacitation in
0.3% albumin was maximal after 6.5 hours, and penetration scores in five of the six donors declined thereafter. In 3.5% albumin, capacitation was slower with
peak penetration levels observed at 13.5 hours. Much
higher penetration was noted when sperm, capacitated
in 3.5% albumin, were compared with those exposed to
0.3%. Specific recommendations for optimizing bioassay outcome are summarized.
Key words: hamster egg bioassay, human sperm, infertility, zona-free
Accepted on July 21, 1982
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