| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
1 Department of Clinical Studies,
New Bolton Center, University of
Pennsylvania School of Veterinary
Medicine, Kennett Square, Pennsylvania,
and the Department of Obstetrics and
Gynecology, University of Pennsylvania
School of Medicine,
Philadelphia, Pennsylvania
The objective was to obtain in vitro sperm capacitation and fertilization to approximate the normal in vivo
processes. Rabbit oocytes from follicles, ovarian surfaces, and oviducts were fertilized in vitro with ejaculated spermatozoa which were treated with high ionic
strength followed by preincubation in defined conditions for 12 to 22 hours. Five bucks were studied and, in
general, high sperm motility scores favored high levels
of fertilization. In all, 305 (55%) of 553 oocytes were
fertilized and 244 (80% of those fertilized) developed to
the four-cell stage within 24 hours; early blastocysts
developed in culture. Transfer of 39 four-cell embryos
into three recipients resulted in seven live young.
Marked improvement both in percentages of fertilization and in four-cell stage development within 24 hours
followed the transfer of oocytes from sperm suspensions after 6 hours. In vitro capacitated spermatozoa
fertilized 62% of ova in vivo after tubal insemination 13
hours after Human Chorionic Gonadotropin (HCG).
Thus, in vitro capacitation and in vitro fertilization approximate the normal in vivo events.
Key words: sperm capacitation, in vitro fertilization, embryo culture, embryo transfer, spermatozoa, oocytes, rabbit, normal development of offspring, high ionic strength treatment, defined medium
Accepted on July 2, 1982
This article has been cited by other articles:
 |
|
 |
|
  J. Tesarik and C. Mendoza Using the Male Gamete for Assisted Reproduction: Past, Present, and Future J Androl, May 1, 2003; 24(3): 317 - 328. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
