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1 Department of Physiology,
Southern Illinois University at Carbondale
2 Department of Physiology,
Southern Illinois University at CarbondaleEngineering Science
Department, California Institute
of Technology, Pasadena, California
A series of semen samples that had been recorded on
motion picture film, then evaluated via standard visual
techniques by a number of well established fertility
clinics (Van Duijn et al (1971)), was analyzed frame-by-frame for sperm motility ratings with the aid of
a movie reader-computer image analysis system. Results of the latter analysis were considered an objective
norm. Statistical comparison of the subjective and objective sets of data revealed that sperm % motility could
be subjectively rated with reasonable accuracy when
the percentage of sperm moving progressively at least
30 µm/second-1 was outside the range of 34-57. Within
this range, however, there is a better than 93% chance
for an erroneous subjective assessment. When the results of the computer method were compared with
those of the spectrophotometric method of Atherton
(1975), a poor correlation was obtained, indicating that
if the latter method is an accurate measure of motility, it
does not discriminate progressive motility.
Key words: sperm motility, statistical comparison, computer analysis
Submitted on October 13, 1980
Revised on June 3, 1981
Accepted on September 28, 1981
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