Journal of Andrology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Published-Ahead-of-Print October 3, 2007, DOI:10.2164/jandrol.107.002998
Journal of Andrology, Vol. 29, No. 1, January/February 2008
Copyright © American Society of Andrology
DOI: 10.2164/jandrol.107.002998

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
29/1/106    most recent
Author Manuscript (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Collodel, G.
Right arrow Articles by Moretti, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Collodel, G.
Right arrow Articles by Moretti, E.

Morphology and Meiotic Segregation in Spermatozoa From Men of Proven Fertility

GIULIA COLLODEL AND ELENA MORETTI

Department of Surgery, Biology Section; Interdepartmental Centre for Research and Therapy of Male Infertility, University of Siena, Siena, Italy.

Correspondence to: Giulia Collodel, Department of Surgery, Biology Section, University of Siena, Policlinico Le Scotte, Viale Bracci, 14, 53100 Siena, Italy (e-mail: collodel{at}unisi.it).


Estimates of semen parameters are important for defining normal ranges, which are currently established by 1999 World Health Organization guidelines. However, it is well known that semen evaluation is questionable because it is necessary for only 1 sperm to be able to reach and fertilize the oocyte. Spermiogram parameters and sperm morphology, evaluated by transmission electron microscopy (TEM), were performed on semen samples from 25 men of proven fertility. Despite a generally normal sperm concentration, progressive motility was reduced in 9 cases. Sperm characteristics were evaluated with an established technique, and the mean of the percentages of sperm pathologies were confirmed by comparing to previous reports. A comparison of apoptosis and necrosis in the samples, as detected by TEM and an annexin V/propidium iodide assay, was also performed. Fluorescence in situ hybridization was carried out on the same samples using probes for chromosomes 18, X, and Y. The mean value of the frequency of total aneuploidy in the analyzed group was 0.627% (25th percentile = 0.563%; median = 0.625%; 75th percentile = 0.690%). This study of the incidence of disomy and diploidy in spermatozoa from fertile, apparently normal individuals is important for making comparisons with infertile cohorts to determine the real increase of aneuploidy in those cohorts.

     Key words: Fertile donors, sperm, TEM, FISH.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2008 by The American Society of Andrology.