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Differential Expression of Neurotrophins in Penises of Streptozotocin–Induced Diabetic Rats

RUN WANG

From the ^* Department of Urology, Affiliated Drum Tower Hospital, Nanjing University, School of Medicine, Nanjing, Jiangsu, China; and the Department of Urology, University of Texas Medical School at Houston and MD Anderson Cancer Center, Houston, Texas.

Correspondence to: Dr Yutian Dai, Department of Urology, Affiliated Drum Tower Hospital, Nanjing University, School of Medicine, Nanjing 210008, China (e-mail: ytdai{at}hotmail.com) or Dr Run Wang, Division of Urology, University of Texas Medical School at Houston, 6431 Fannin, MSB 6.018, Houston, TX 77030 (e-mail: Run.Wang{at}uth.tmc.edu).

To explore the mechanism of diabetic erectile dysfunction, we studied the distribution of neurotrophins in the penises of diabetic rats, including nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). Male Sprague-Dawley rats were injected with 65 mg/kg streptozotocin to induce diabetes mellitus (DM). The control rats were raised as age-matched control. Eight weeks later, the intercavernous pressure (ICP) of the rats was measured after electrostimulation and before sacrifice. Each peeled penis was divided into 2 parts, one for immunohistochemistry and the other for Western blot analysis. The ICP of the DM group rats was significantly decreased as compared to the vehicle control rats. There were significantly more NGF-positive neurons in the penises of the diabetic rats than in those of the control rats, while the opposite results were observed for BDNF-positive neurons. In the Western blot analysis, the proteins of NGF, NT-3, and NT-4 were all increased, while that of BDNF was decreased in diabetic rats. This is the first study revealing the expression of NT-4 protein in cavernous tissue. The abnormal level of these 4 neurotrophins in cavernous tissue may be one of the factors of the pathogenesis of diabetic ED. The increase of neurotrophins may reflect the degree of cavernous tissue denervation and may represent a compensatory mechanism. The lesion of the retrograde axonal transport of the nerves caused by hyperglycemia may be related to this phenomenon.

     Key words: Diabetes mellitus, erectile dysfunction, intercavernous pressure