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From * Andrology and IVF Laboratories, University
of Utah School of Medicine, Salt Lake City, Utah; and
Reproductive Medicine Center, University of
Minnesota, Minneapolis, Minnesota.
| Correspondence to: Dr Douglas T. Carrell, Associate Professor of Surgery (Urology), OB-GYN and Physiology, Director of Andrology and IVF Laboratories, University of Utah School of Medicine, 675 Arapeen Dr, Suite 205, Salt Lake City, UT 84108 (e-mail: douglas.carrell{at}hsc.utah.edu). |
15%, >15%-<30%, and 
30%) showed
that the SCSA, TUNEL, and SCD test predict the same levels of DNA
fragmentation. AOT consistently showed higher levels of DNA fragmentation for
each DFI category. DNA fragmentation in sperm between infertile men and donor
sperm was significantly different (P < .05) under SCSA (22.0 ± 1.6
vs 11.8 ± 1.4), TUNEL (19.5 ± 1.3 vs 11.1 ± 0.9) and SCD
(20.4 ± 1.3 vs 10.8 ± 1.1), respectively. DNA fragmentation in
sperm evaluated by AOT did not differ (P > .05) between infertile men (31.3
± 2.4) and donors (32.7 ± 4.8). AOT showed extreme variations
for sperm DNA fragmentation in semen from both infertile men and donors. The
problems of indistinct colors, rapid fading, and the heterogeneous staining
were also faced. In conclusion, SCSA, TUNEL, and SCD show similar predictive
values for DNA fragmentation, and AOT shows variable and increased levels of
DNA fragmentation, which makes it of questionable value in clinical
practice.
Key words: SCSA, TUNEL, SCD, AOT
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