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Journal of Andrology, Vol. 26, No. 2, March/April 2005
Copyright © American Society of Andrology

Novel Development-Related Alternative Splices in Human Testis Identified by cDNA Microarrays

XIAOYAN HUANG, JIANMIN LI, LI LU, MIN XU, JUNHUA XIAO, LANLAN YIN, HU ZHU, ZUOMIN ZHOU AND JIAHAO SHA

From the Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, People's Republic of China.

Correspondence to: Dr Jiahao Sha, Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 210029, People's Republic of China (e-mail: shajh{at}njmu.edu.cn).


Alternative splicing of premessenger RNA is an important regulatory mechanism that increases the diversity of proteins transcribed from a single gene. This is particularly important in the testis because germ cell expansion and differentiation require many cellular changes and regulatory steps. To investigate novel development-related alternative splicings in the human testis, complementary DNA microarray studies were conducted with the use of probes from human fetal testes, adult testes, and human spermatozoa. Of a total of 386 Unigene clusters found to be related to the development of the testis, 67 clusters showed a total of 74 novel alternative spliceoforms. Developmental stage-dependent expression was also performed for a novel Unigene, NYD-SP20 (Hs.351068), which had 4 possible novel spliceoforms and another Unigene, CRISP2 (cysteine-rich secretory protein 2, Hs.2042), which had 3 possible novel spliceoforms. These results indicate that alternative splicing plays an important role in the complicated processes of testis development and spermatogenesis.

     Key words: Alternative splicing, spermatogenesis, regulatory mechanism, protein diversity, spliceoforms




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