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A Suite of Novel Human Spermatozoal RNAs

G. CHARLES OSTERMEIER^*,

ROBERT J. GOODRICH^*,

STEPHEN A. KRAWETZ^*,,

From the ^* Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, and Center for Scientific Computing, Wayne State University, Detroit, Michigan.

Correspondence to: Stephen A. Krawetz, Charlotte B. Failing Professor of Fetal Therapy and Diagnosis, 253 C.S. Mott Center, 275 East Hancock, Detroit, MI 48201 (e-mail: steve{at}compbio.med.wayne.edu).

We recently described a complex population of spermatozoal coding RNAs that are delivered to the oocyte on fertilization. These are derived throughout spermatogenesis, representing a record of past events. Recently, evidence has been provided that micro-RNAs are present in testes, suggesting that they might also be carried in ejaculate spermatozoa. To directly test this hypothesis, a unique microarray system capable of directly identifying antisense RNAs and predicted transcripts was utilized. RNA isolated from the ejaculate spermatozoa of 6 normal fertile men was directly hybridized to sense oligonucleotide arrays containing 10 000 elements. This revealed 68 shared RNAs, some of which are similar to those previously defined as micro-RNAs, whereas others were the antisense of previously in silico-predicted transcripts. The results and implications of this study are described in this communication.

     Key words: Development, fertility, cloning, microarray, novel transcripts, antisense RNA, spermatozoa.

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