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Gene and Protein Profiling of the Response of MA-10 Leydig Tumor Cells to Human Chorionic Gonadotropin

HAKIMA AMRI

HONGZHAN HUANG^*,

CATHY WU^*,

From the ^* Departments of Biochemistry and Molecular Biology, Physiology and Biophysics, and Protein Information Resource, Georgetown University Medical Center, Washington, DC.

Correspondence to: V. Papadopoulos, Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Basic Sciences Bldg, 3900 Reservoir Rd, Washington, DC 20057 (e-mail: papadopv{at}georgetown.edu).

Activation of the steroidogenic machinery by peptide hormones involves a number of steps for transmitting signals from the plasma membrane to mitochondria in a spatially and temporally coordinated manner. Although key proteins mediating the hormonal signal have been identified, recent data suggest that the pathway might involve more complex protein-protein and protein-lipid interactions. Genomic and proteomic methods of analysis, namely the Affymetrix Murine Genome U74A v2 GeneChip and the BD PowerBlot Western Array, were used to identify human chorionic gonadotropin (hCG)-induced changes in mRNA and protein of MA-10 Leydig tumor cells that parallel the increase seen in progesterone synthesis. To analyze the massive amount of data that was generated, a comprehensive protein information matrix summarizing the features of each gene or protein, including its known properties, as well as annotations derived by homology-based functional inference, was developed. Of the genes examined by Affymetrix array, approximately 79 were differentially expressed and of gene products examined by PowerBlot, 9 were differentially expressed (above twofold). Changes in the expression of selected transcripts of interest were confirmed using real-time quantitative polymerase chain reaction and immunoblot analyses. Collectively, these results indicate that hormonal regulation of steroidogenesis is a complex phenomenon, involving proteins that participate in various known and novel pathways, which are implicated in transmitting signals from the plasma membrane to mitochondria and nucleus.

     Key words: Testis, gene ontology, genomics, proteomics, steroidogenesis

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