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Journal of Andrology, Vol. 24, No. 2, March/April 2003
Copyright © American Society of Andrology

Deterioration of Plasma Membrane Is Associated With Activated Caspases in Human Spermatozoa

UWE PAASCH*, SONJA GRUNEWALD*, GUENTER FITZL{dagger} AND HANS-JUERGEN GLANDER*

From the * Department of Dermatology/Andrology Unit, and {dagger} Department of Anatomy, University of Leipzig, Leipzig, Germany.

Correspondence to: Hans-Juergen Glander, Department of Dermatology/Andrology Unit, University of Leipzig, Stephanstrasse 11, D-04103 Leipzig, Germany (e-mail: glah{at}medizin.uni-leipzig.de).


Spermatozoa with deteriorated plasma membranes can be separated by magnetic-activated cell sorting (MACS) after binding superparamagnetic annexin V-conjugated microbeads (ANMBs) to membrane phosphatidylserine (PS). Semen samples from 15 donors and 25 infertile patients were divided into 2 spermatozoal fractions by annexin V-MACS. Activated caspases (aCPs), which mediate degradations of cell quality, were determined by CaspaTag in the 2 subpopulations. Spermatozoa from donors showed lower levels of bound annexin V (3.6% ± 0.5% vs 11.9% ± 1.1%; P < .01) and aCPs (21.8% ± 2.6% vs 43.2% ± 2.1%; P < .01) than did spermatozoa from infertile patients. MACS resulted in a decrease of spermatozoa with aCPs from 21.8% ± 2.6% (before separation) to 9.2% ± 1.4% (in the ANMB-negative fraction) in donors and from 43.2% ± 2.1% to 18.8% ± 2.6% in infertile patients (mean ± SEM; P < .01). Separation effects of the MACS technique were confirmed with flow cytometry using anti-annexin V antibodies and with electron microscopy. ANMB-MACS removes spermatozoa with PS-bound annexin V and produces a higher quality spermatozoal fraction. Spermatozoa with a deteriorated membrane are characterized by an increase in aCPs. A higher percentage of spermatozoa with ANMBs bound to PS and with aCPs were found in infertile patients.

     Key words: Annexin V, immunomagnetic cell depletion, human spermatozoa, caspases, electron microscopy




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