Journal of Andrology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bureau, M.
Right arrow Articles by Sirard, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bureau, M.
Right arrow Articles by Sirard, M. A.

Journal of Andrology, Vol 23, Issue 2 188-193, Copyright © 2002 by The American Society of Andrology

JOURNAL ARTICLE

Binding regulation of porcine spermatozoa to oviductal vesicles in vitro

M. Bureau, J. L. Bailey and M. A. Sirard
Centre de Recherche en Biologie de la Reproduction, Departement des Sciences Animales, Universite Laval, Ste-Foy, Quebec, Canada.

In vivo, en route to the site of fertilization, mammalian spermatozoa bind to oviduct epithelial cells (OECs). This binding may favor sperm survival and capacitation, but little is known about the regulation of detachment of sperm from the oviduct in vivo. Therefore, we studied sperm-oviduct interaction in vitro using vesicles formed from OECs in primary culture. Porcine oviducts were collected from gilts at the slaughterhouse. OECs were obtained by compressing the oviduct and culturing them in TCM-199 medium with 10% fetal calf serum for 48 hours. For the first experiment, to test the hypothesis that progesterone (P4) and estradiol (E2) affect sperm-OEC binding, OECs were pretreated for 48 hours with 100 ng/mL of P4 or E2. In the second experiment, porcine follicular fluid (pFF, 5%), caffeine (1 microM), calcium ionophore A23187 (1 microM), and DMSO (0.01%) were added to the incubation medium to provide insights on the mechanisms of sperm release from the oviduct. For both experiments, 50 x 10(6) sperm were coincubated with 50 microL of OEC vesicles in 1 mL of incubation medium for up to 24 hours at 37 degrees C and 5% CO2. After an initial 30 minutes of coincubation, the vesicles settled and a sample of the supernatant was removed to evaluate sperm release and acrosomal status; subsequent samples were removed after 2, 4, and 24 hours of coincubation. To evaluate the effect of the different treatments on sperm integrity, the acrosome status of the spermatozoa was determined using fluoresceinlabeled Pisum sativum agglutinin staining. Experiment 1 showed that P4 pretreatment of OECs interferes with sperm binding compared with pretreatment with E2 or controls (P < .05). In experiment 2, coincubation in the presence of A23187 increased sperm detachment compared with pretreatment with DMSO, pFF, caffeine, or controls (P < .05). For each experiment, the treatments did not affect the percentage of acrosome-reacted sperm compared with controls (P < .05).


This article has been cited by other articles:


Home page
 Toxicol SciHome page
S. Hombach-Klonisch, P. Pocar, J. Kauffold, and T. Klonisch
Dioxin Exerts Anti-estrogenic Actions in a Novel Dioxin-Responsive Telomerase-Immortalized Epithelial Cell Line of the Porcine Oviduct (TERT-OPEC)
Toxicol. Sci., April 1, 2006; 90(2): 519 - 528.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by The American Society of Andrology.