| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Journal of Andrology, Vol 18, Issue 1 80-87, Copyright © 1997 by The American Society of Andrology
JOURNAL ARTICLE |
M. L. Meistrich and M. Kangasniemi
Department of Experimental Radiation Oncology, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
The possibility of stimulating the recovery of spermatogenesis after irradiation using hormone treatment was tested in LBNF, rats. At 10 weeks after irradiation with 3.5 Gy, the percentage of tubules showing recovery of spermatogenesis (repopulation index) was 37% in rats that received no hormone treatment. GnRH agonist (GnRH-Ag) treatment with Zoladex or continuous treatment with testosterone markedly stimulated the recovery of spermatogenesis. When GnRH-Ag treatment was started immediately after 3.5-Gy irradiation and maintained for 10 weeks, the repopulation index was 91%. When an additional 6.5 weeks without further treatment was allowed between the 10-week GnRH treatment and killing the rats, the repopulation index recovered to 100% and sperm counts to 83 x 10(6). These sperm counts were more than 100-fold higher than those in rats not given hormone treatment and 50% of normal nonirradiated control levels. GnRH-Ag for 10 weeks also stimulated spermatogenic recovery in rats irradiated with 6 Gy, even when the start of treatment was delayed until 18 weeks after irradiation. Without GnRH-Ag, the repopulation index was 0, but in GnRH-Ag-treated rats it was 14.5%. Since all of the hormone treatments suppress intratesticular testosterone, high levels of testosterone may be inhibiting differentiation and their suppression may stimulate recovery. Even though the exact mechanism is not yet known, this method may still be applicable for clinical use to activate spermatogenesis in patients rendered azoospermic by irradiation or possibly by other cytotoxic treatments.
This article has been cited by other articles:
 |
|
 |
|
  Z. Zhang, S. Shao, and M. L. Meistrich Irradiated Mouse Testes Efficiently Support Spermatogenesis Derived From Donor Germ Cells of Mice and Rats J Androl, May 1, 2006; 27(3): 365 - 375. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Porter, G. Shetty, and M. L. Meistrich Testicular Edema Is Associated with Spermatogonial Arrest in Irradiated Rats Endocrinology, March 1, 2006; 147(3): 1297 - 1305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Shetty, C. C. Y. Weng, S. J. Meachem, O. U. Bolden-Tiller, Z. Zhang, P. Pakarinen, I. Huhtaniemi, and M. L. Meistrich Both Testosterone and Follicle-Stimulating Hormone Independently Inhibit Spermatogonial Differentiation in Irradiated Rats Endocrinology, January 1, 2006; 147(1): 472 - 482. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Solakidi, A-M.G. Psarra, S. Nikolaropoulos, and C.E. Sekeris Estrogen receptors {alpha} and {beta} (ER{alpha} and ER{beta}) and androgen receptor (AR) in human sperm: localization of ER{beta} and AR in mitochondria of the midpiece Hum. Reprod., December 1, 2005; 20(12): 3481 - 3487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Matthiesson, P. G. Stanton, L. O'Donnell, S. J. Meachem, J. K. Amory, R. Berger, W. J. Bremner, and R. I. McLachlan Effects of Testosterone and Levonorgestrel Combined with a 5{alpha}-Reductase Inhibitor or Gonadotropin-Releasing Hormone Antagonist on Spermatogenesis and Intratesticular Steroid Levels in Normal Men J. Clin. Endocrinol. Metab., October 1, 2005; 90(10): 5647 - 5655. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J Meachem, D. M Robertson, N. G Wreford, R. I McLachlan, and P. G Stanton Oestrogen does not affect the restoration of spermatogenesis in the gonadotrophin-releasing hormone-immunised adult rat J. Endocrinol., June 1, 2005; 185(3): 529 - 538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Boekelheide, H. A. Schoenfeld, S. J. Hall, C. C. Weng, G. Shetty, J. Leith, J. Harper, M. Sigman, D. L. Hess, and M. L. Meistrich Gonadotropin-Releasing Hormone Antagonist (Cetrorelix) Therapy Fails to Protect Nonhuman Primates (Macaca arctoides) From Radiation-Induced Spermatogenic Failure J Androl, March 1, 2005; 26(2): 222 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Shetty and M. L. Meistrich Hormonal Approaches to Preservation and Restoration of Male Fertility After Cancer Treatment J Natl Cancer Inst Monographs, March 1, 2005; 2005(34): 36 - 39. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Shetty, C. C. Y. Weng, O. U. Bolden-Tiller, I. Huhtaniemi, D. J. Handelsman, and M. L. Meistrich Effects of Medroxyprogesterone and Estradiol on the Recovery of Spermatogenesis in Irradiated Rats Endocrinology, October 1, 2004; 145(10): 4461 - 4469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kanatsu-Shinohara, T. Morimoto, S. Toyokuni, and T. Shinohara Regulation of Mouse Spermatogonial Stem Cell Self-Renewing Divisionby the Pituitary Gland Biol Reprod, June 1, 2004; 70(6): 1731 - 1737. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Meistrich, G. Wilson, K. L. Porter, I. Huhtaniemi, G. Shetty, and G. A. Shuttlesworth Restoration of Spermatogenesis in Dibromochloropropane (DBCP)-Treated Rats by Hormone Suppression Toxicol. Sci., December 1, 2003; 76(2): 418 - 426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ohmura, T. Ogawa, M. Ono, M. Dezawa, M. Hosaka, Y. Kubota, and H. Sawada Increment of Murine Spermatogonial Cell Number by Gonadotropin-Releasing Hormone Analogue Is Independent of Stem Cell Factor c-kit Signal Biol Reprod, June 1, 2003; 68(6): 2304 - 2313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Meistrich and G. Shetty Inhibition of Spermatogonial Differentiation by Testosterone J Androl, March 1, 2003; 24(2): 135 - 148. [Full Text] [PDF]
|
|
|
|
|
|
G. Shetty, G. Wilson, M. P. Hardy, E. Niu, I. Huhtaniemi, and M. L. Meistrich Inhibition of Recovery of Spermatogenesis in Irradiated Rats by Different Androgens Endocrinology, September 1, 2002; 143(9): 3385 - 3396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.B. Thomson, R.A. Anderson, D.S. Irvine, C.J.H. Kelnar, R.M. Sharpe, and W.H.B. Wallace Investigation of suppression of the hypothalamic-pituitary-gonadal axis to restore spermatogenesis in azoospermic men treated for childhood cancer Hum. Reprod., July 1, 2002; 17(7): 1715 - 1723. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.I. McLachlan, L. O'Donnell, S.J. Meachem, P.G. Stanton, D.M. de Kretser, K. Pratis, and D.M. Robertson Identification of Specific Sites of Hormonal Regulation in Spermatogenesis in Rats, Monkeys, and Man Recent Prog. Horm. Res., January 1, 2002; 57(1): 149 - 179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tohda, K. Matsumiya, Y. Tadokoro, K. Yomogida, Y. Miyagawa, K. Dohmae, A. Okuyama, and Y. Nishimune Testosterone Suppresses Spermatogenesis in Juvenile Spermatogonial Depletion (jsd ) Mice Biol Reprod, August 1, 2001; 65(2): 532 - 537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Shetty, G. Wilson, I. Huhtaniemi, H. Boettger-Tong, and M. L. Meistrich Testosterone Inhibits Spermatogonial Differentiation in Juvenile Spermatogonial Depletion Mice Endocrinology, July 1, 2001; 142(7): 2789 - 2795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Maiti, M. L. Meistrich, G. Wilson, G. Shetty, M. Marcelli, M. J. McPhaul, P. L. Morris, and M. F. Wilkinson Irradiation Selectively Inhibits Expression from the Androgen-Dependent Pem Homeobox Gene Promoter in Sertoli Cells Endocrinology, April 1, 2001; 142(4): 1567 - 1577. [Abstract] [Full Text]
|
|
|
|
|
|
H. A. Schoenfeld, S. J. Hall, and K. Boekelheide Continuously Proliferative Stem Germ Cells Partially Repopulate the Aged, Atrophic Rat Testis after Gonadotropin-Releasing Hormone Agonist Therapy Biol Reprod, April 1, 2001; 64(4): 1273 - 1282. [Abstract] [Full Text]
|
|
|
|
|
|
G. Shetty, G. Wilson, I. Huhtaniemi, G. A. Shuttlesworth, T. Reissmann, and M. L. Meistrich Gonadotropin-Releasing Hormone Analogs Stimulate and Testosterone Inhibits the Recovery of Spermatogenesis in Irradiated Rats Endocrinology, May 1, 2000; 141(5): 1735 - 1745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Shuttlesworth, D. G. De Rooij, I. Huhtaniemi, T. Reissmann, L. D. Russell, G. Shetty, G. Wilson, and M. L. Meistrich Enhancement of A Spermatogonial Proliferation and Differentiation in Irradiated Rats by Gonadotropin-Releasing Hormone Antagonist Administration Endocrinology, January 1, 2000; 141(1): 37 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. de Rooij, M. Okabe, and Y. Nishimune Arrest of Spermatogonial Differentiation in jsd/jsd, Sl17H/Sl17H, and Cryptorchid Mice Biol Reprod, September 1, 1999; 61(3): 842 - 847. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. L. Meistrich, G. Wilson, and I. Huhtaniemi Hormonal Treatment after Cytotoxic Therapy Stimulates Recovery of Spermatogenesis Cancer Res., August 1, 1999; 59(15): 3557 - 3560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Schlatt, G. Rosiepen, G.F. Weinbauer, C. Rolf, P.F. Brook, and E. Nieschlag Germ cell transfer into rat, bovine, monkey and human testes Hum. Reprod., January 1, 1999; 14(1): 144 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. T. Blanchard, J. Lee, and K. Boekelheide Leuprolide, a Gonadotropin-Releasing Hormone Agonist, Reestablishes Spermatogenesis After 2,5-Hexanedione-Induced Irreversible Testicular Injury in the Rat, Resulting in Normalized Stem Cell Factor Expression Endocrinology, January 1, 1998; 139(1): 236 - 244. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
