| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Journal of Andrology, Vol 17, Issue 4 382-393, Copyright © 1996 by The American Society of Andrology
JOURNAL ARTICLE |
J. Singh and D. J. Handelsman
Andrology Unit, Royal Prince Alfred Hospital, Sydney, NSW, Australia.
In order to clarify the mechanism of synergism between follicle-stimulatory hormone(FSH) and testosterone (T) in the hormonal regulation of spermatogenesis, we studied the effects of recombinant human FSH (rhFSH) on the induction of spermatogenesis by testosterone in congenitally gonadotropin-deficient hpg mice. Weanling (day 21) homozygous hpg mice were administered daily subcutaneous injections of 1, 5, or 10 IU of rhFSH alone or in combination with a subdermal testosterone implant until day 70 of age. Spermatogenesis was quantitated by stereological estimation of germ cell populations and Sertoli cells as well as measurement of diameter of seminiferous tubules and their lumina in perfusion-fixed testes and by counting homogenization-resistant condensed testicular spermatids. Recombinant human FSH alone increased the absolute numbers of spermatogonia (x3.5-fold) and spermatocytes (x3-fold) compared with untreated hpg mice but did not significantly increase Sertoli cell numbers or form any condensed spermatids or tubular lumen. Relative to Sertoli cell numbers, rhFSH alone increased populations of spermatogonia (x2-fold) and spermatocytes (x2-fold). The addition of T to rhFSH further increased the absolute numbers of spermatogonia (x5-fold) and spermatocytes (x3.5-fold) compared with untreated hpg mice as well as increasing tubular diameter and forming tubular lumina. In addition administration of T allowed the completion of spermatogenesis in the presence of intratesticular T levels that were approximately 2% of non-hpg controls. All effects of the FSH + T combination were however no greater than the effects of the equivalent dose of T alone. The present study therefore indicates that rhFSH alone increases proliferation of premeiotic spermatogenic cells but has no effect on the completion of spermiogenesis or on Sertoli cell maturation. Furthermore we were unable to identify any additive effects of FSH with T in the hormonal regulation of spermatogenesis in the hpg mouse. This suggests that FSH or T both may stimulate initial spermatogenic development, but only T can complete spermiogenesis.
This article has been cited by other articles:
 |
|
 |
|
  D. R. Goldman-Johnson, D. M. de Kretser, and J. R. Morrison Evidence that Androgens Regulate Early Developmental Events, Prior to Sexual Differentiation Endocrinology, January 1, 2008; 149(1): 5 - 14. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. E Chausiaux, M. H Abel, F. O Baxter, W. T Khaled, P. J.I Ellis, H. M Charlton, and N. A Affara Hypogonadal Mouse, a Model to Study the Effects of the Endogenous Lack of Gonadotropins on Apoptosis Biol Reprod, January 1, 2008; 78(1): 77 - 90. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-Y. Tsai, S.-D. Yeh, R.-S. Wang, S. Yeh, C. Zhang, H.-Y. Lin, C.-R. Tzeng, and C. Chang Differential effects of spermatogenesis and fertility in mice lacking androgen receptor in individual testis cells PNAS, December 12, 2006; 103(50): 18975 - 18980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Shetty, C. C. Y. Weng, K. L. Porter, Z. Zhang, P. Pakarinen, T. R. Kumar, and M. L. Meistrich Spermatogonial Differentiation in Juvenile Spermatogonial Depletion (jsd) Mice with Androgen Receptor or Follicle-Stimulating Hormone Mutations Endocrinology, July 1, 2006; 147(7): 3563 - 3570. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R Rathi, A Honaramooz, W Zeng, R Turner, and I Dobrinski Germ cell development in equine testis tissue xenografted into mice. Reproduction, June 1, 2006; 131(6): 1091 - 1098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Baines, M. O Nwagwu, E. C Furneaux, J. Stewart, J. B Kerr, T. M Mayhew, and F. J P Ebling Estrogenic induction of spermatogenesis in the hypogonadal (hpg) mouse: role of androgens Reproduction, November 1, 2005; 130(5): 643 - 654. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, H. Newton, J. A. Spaliviero, C. M. Allan, B. Marshan, D. J. Handelsman, and P. J. Illingworth Gonadotropin Control of Inhibin Secretion and the Relationship to Follicle Type and Number in the hpg Mouse Biol Reprod, October 1, 2005; 73(4): 610 - 618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sofikitis, E. Pappas, A. Kawatani, D. Baltogiannis, D. Loutradis, N. Kanakas, D. Giannakis, F. Dimitriadis, K. Tsoukanelis, I. Georgiou, et al. Efforts to create an artificial testis: culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment Hum. Reprod. Update, May 1, 2005; 11(3): 229 - 259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Allan, A. Garcia, J. Spaliviero, F.-P. Zhang, M. Jimenez, I. Huhtaniemi, and D. J. Handelsman Complete Sertoli Cell Proliferation Induced by Follicle-Stimulating Hormone (FSH) Independently of Luteinizing Hormone Activity: Evidence from Genetic Models of Isolated FSH Action Endocrinology, April 1, 2004; 145(4): 1587 - 1593. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. W. Holdcraft and R. E. Braun Androgen receptor function is required in Sertoli cells for the terminal differentiation of haploid spermatids Development, January 15, 2004; 131(2): 459 - 467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Spaliviero, M. Jimenez, C. M. Allan, and D. J. Handelsman Luteinizing Hormone Receptor-Mediated Effects on Initiation of Spermatogenesis in Gonadotropin-Deficient (hpg) Mice Are Replicated by Testosterone Biol Reprod, January 1, 2004; 70(1): 32 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Haywood, J. Spaliviero, M. Jimemez, N. J. C. King, D. J. Handelsman, and C. M. Allan Sertoli and Germ Cell Development in Hypogonadal (hpg) Mice Expressing Transgenic Follicle-Stimulating Hormone Alone or in Combination with Testosterone Endocrinology, February 1, 2003; 144(2): 509 - 517. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Singh, L. Young, D. J. Handelsman, and Q. Dong Prostate Epithelial Expression of a Novel Androgen Target Gene J Androl, September 1, 2002; 23(5): 652 - 660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tohda, K. Matsumiya, Y. Tadokoro, K. Yomogida, Y. Miyagawa, K. Dohmae, A. Okuyama, and Y. Nishimune Testosterone Suppresses Spermatogenesis in Juvenile Spermatogonial Depletion (jsd ) Mice Biol Reprod, August 1, 2001; 65(2): 532 - 537. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. O'Donnell, K. M. Robertson, M. E. Jones, and E. R. Simpson Estrogen and Spermatogenesis Endocr. Rev., June 1, 2001; 22(3): 289 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Allan, M. Haywood, S. Swaraj, J. Spaliviero, A. Koch, M. Jimenez, M. Poutanen, J. Levallet, I. Huhtaniemi, P. Illingworth, et al. A Novel Transgenic Model to Characterize the Specific Effects of Follicle-Stimulating Hormone on Gonadal Physiology in the Absence of Luteinizing Hormone Actions Endocrinology, June 1, 2001; 142(6): 2213 - 2220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F.-P. Zhang, M. Poutanen, J. Wilbertz, and I. Huhtaniemi Normal Prenatal but Arrested Postnatal Sexual Development of Luteinizing Hormone Receptor Knockout (LuRKO) Mice Mol. Endocrinol., January 1, 2001; 15(1): 172 - 183. [Abstract] [Full Text]
|
|
|
|
|
|
F. J. P. Ebling, A. N. Brooks, A. S. Cronin, H. Ford, and J. B. Kerr Estrogenic Induction of Spermatogenesis in the Hypogonadal Mouse Endocrinology, August 1, 2000; 141(8): 2861 - 2869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Handelsman, J. A. Spaliviero, J. M. Simpson, C. M. Allan, and J. Singh Spermatogenesis without Gonadotropins: Maintenance Has a Lower Testosterone Threshold than Initiation Endocrinology, September 1, 1999; 140(9): 3938 - 3946. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
