Journal of Andrology Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hayes, R.
Right arrow Articles by Hedger, M. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hayes, R.
Right arrow Articles by Hedger, M. P.

Journal of Andrology, Vol 17, Issue 1 41-49, Copyright © 1996 by The American Society of Andrology

JOURNAL ARTICLE

Secretion of bioactive interleukin 1 by rat testicular macrophages in vitro

R. Hayes, S. A. Chalmers, D. J. Nikolic-Paterson, R. C. Atkins and M. P. Hedger
Institute of Reproduction and Development, Monash University, Clayton, Victoria, Australia.

The relative capacities for testicular macrophages and resident peritoneal macrophages to secrete the pro-inflammatory cytokine, interleukin 1 (LL-1), in response to stimulation by bacterial lipopolysaccharide (LPS), were compared in vitro. Macrophages were isolated from adult male rat testicular interstitial cells or peritoneal lavage by adherence to glass coverslips or plastic culture dishes. The macrophages were immediately cultured, with or without a maximal dose of LPS (1 mu g/ml), over 24 hours at 32 degrees Celsius. Bioactive LL-1 production was measured by a sensitive thymocyte proliferation bioassay, employing recombinant human LL-1 beta as the reference standard. In comparison with the peritoneal macrophages, testicular macrophages displayed only a very small response to LPS, producing 2.8% of the amount of LL-1 per cell secreted by peritoneal macrophages cultured under identical conditions. Production of authentic LL-1 was confirmed by inhibition of the bioassay response in the presence of human recombinant LL-1 receptor antagonist. A small molecular mass (<10 kDa based on ultrafiltration) inhibitor of LL-1 bioactivity was also present in the medium collected from both cultures, but this inhibitory activity did not account for the differences in activity observed. In cultures of total peritoneal cells under similar conditions, addition of testosterone (10-1,000 ng/ml) did not affect LL-1 production in response to LPS. These data indicate that testicular macrophages have a reduced ability to secrete bioactive IL-1, and they provide further evidence for an altered capacity for immune responses within the testis.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
S. Bhushan, S. Tchatalbachev, J. Klug, M. Fijak, C. Pineau, T. Chakraborty, and A. Meinhardt
Uropathogenic Escherichia coli Block MyD88-Dependent and Activate MyD88-Independent Signaling Pathways in Rat Testicular Cells
J. Immunol., April 15, 2008; 180(8): 5537 - 5547.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
W. R. Winnall, U. Ali, M. K. O'Bryan, J. J. Hirst, P. A.F. Whiley, J. A. Muir, and M. P. Hedger
Constitutive Expression of Prostaglandin-Endoperoxide Synthase 2 by Somatic and Spermatogenic Cells Is Responsible for Prostaglandin E2 Production in the Adult Rat Testis
Biol Reprod, May 1, 2007; 76(5): 759 - 768.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
M. F. Riera, M. N. Galardo, E. H. Pellizzari, S. B. Meroni, and S. B. Cigorraga
Participation of phosphatidyl inositol 3-kinase/protein kinase B and ERK1/2 pathways in interleukin-1{beta} stimulation of lactate production in Sertoli cells
Reproduction, April 1, 2007; 133(4): 763 - 773.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. K. O'Bryan, O. Gerdprasert, D. J. Nikolic-Paterson, A. Meinhardt, J. A. Muir, L. M. Foulds, D. J. Phillips, D. M. de Kretser, and M. P. Hedger
Cytokine profiles in the testes of rats treated with lipopolysaccharide reveal localized suppression of inflammatory responses
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1744 - R1755.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
V. Morales, P. Santana, R. Diaz, C. Tabraue, G. Gallardo, F. L. Blanco, I. Hernandez, L. F. Fanjul, and C. M. Ruiz de Galarreta
Intratesticular Delivery of Tumor Necrosis Factor-{alpha} and Ceramide Directly Abrogates Steroidogenic Acute Regulatory Protein Expression and Leydig Cell Steroidogenesis in Adult Rats
Endocrinology, November 1, 2003; 144(11): 4763 - 4772.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
F. C. W. Wu and A. von Eckardstein
Androgens and Coronary Artery Disease
Endocr. Rev., April 1, 2003; 24(2): 183 - 217.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
O. Gerdprasert, M.K. O'Bryan, D.J. Nikolic-Paterson, K. Sebire, D.M. de Kretser, and M.P. Hedger
Expression of monocyte chemoattractant protein-1 and macrophage colony-stimulating factor in normal and inflamed rat testis
Mol. Hum. Reprod., June 1, 2002; 8(6): 518 - 524.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A Meinhardt, M Bacher, M. O'Bryan, J. McFarlane, C Mallidis, C Lehmann, C. Metz, D. de Kretser, R Bucala, and M. Hedger
A switch in the cellular localization of macrophage migration inhibitory factor in the rat testis after ethane dimethane sulfonate treatment
J. Cell Sci., January 5, 1999; 112(9): 1337 - 1344.
[Abstract] [PDF]

Home page
 Endocr. Rev.Home page
L. Gnessi, A. Fabbri, and G. Spera
Gonadal Peptides as Mediators of Development and Functional Control of the Testis: An Integrated System with Hormones and Local Environment
Endocr. Rev., August 1, 1997; 18(4): 541 - 609.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1996 by The American Society of Andrology.