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Journal of Andrology, Vol 16, Issue 3 272-277, Copyright © 1995 by The American Society of Andrology

JOURNAL ARTICLE

Acrosin activity of human spermatozoa by means of a simple gelatinolytic technique: a method useful for IVF

R. Henkel, C. Muller, W. Miska, W. B. Schill, J. Kleinstein and H. Gips
Department of Dermatology and Andrology, Justus Liebig University, Giessen, FRG.

Acrosin activity was determined using a gelatinolysis technique in 100-microliter semen aliquots of 114 patients (normozoospermia, n = 90; asthenozoospermia, n = 12; oligozoospermia, n = 10; polyzoospermia, n = 2) attending an in vitro fertilization (IVF) program. Halo diameter, halo formation rate, and a calculated acrosin activity index correlated significantly with the IVF rates (P = 0.0054, r = 0.396; P = 0.0009, r = 0.401; and P = 0.0003, r = 0.428, respectively). In cases where the halo diameter was < 10 microns and halo formation rate was < 60%, all patients were subfertile or infertile, that is, they showed poor or no fertilization in vitro, respectively. The assay demonstrated a relatively low sensitivity: 25.7% for halo diameter, 37.1% for halo formation rate, and 25.7% for acrosin activity index, respectively. This might be attributed to other sperm functional aspects, such as disturbed acrosome reaction or impaired zona binding.




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Copyright © 1995 by The American Society of Andrology.