Journal of Andrology Cross-Journal Searching
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Young, R. J.
Right arrow Articles by Bodt, B. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Young, R. J.
Right arrow Articles by Bodt, B. A.

Journal of Andrology, Vol 15, Issue 4 362-377, Copyright © 1994 by The American Society of Andrology

JOURNAL ARTICLE

Development of computer-directed methods for the identification of hyperactivated motion using motion patterns developed by rabbit sperm during incubation under capacitation conditions

R. J. Young and B. A. Bodt
Life Sciences Department, Edgewood Research, Development and Engineering Center, Aberdeen Proving Ground, Maryland.

Rabbit spermatozoa developed motions that mimicked hyperactivated motility during incubation for 16-20 hours under capacitation conditions and in several other commonly used media. Sperm from some rabbits failed to acquire this type of motility, and sperm from others failed to survive the long incubation time. Four motility patterns developed during incubation for 16-20 hours. Motility parameters measured by the CellSoft and CellTrak motion analysis systems were similar except for the average amplitude of lateral head displacement. Multivariate discriminant analysis with complementary regression analysis, and an unrelated tree structured classification method (CART), were used to derive rules, based on motility parameters, for the objective classification of sperm into the two motility classes: 1) nonhyperactivated motility and 2) hyperactivated motility or motility that mimicked hyperactivated motility. The motility parameter wobble (WOB) as superior to the commonly used parameter, linearity, as a classifier of motility types. It classified sperm into the two motility groups with 96.6% efficiency and, together with curvilinear velocity (VCL), attained classification efficiencies of 98%. The classification model produced by CART was preferred over the one obtained by discriminant analysis. The rule for motility classification was dependent on the motion analysis system used to measure the motion parameters. The rule for the CellSoft system, WOB < or = 0.78 and VCL > or = 51 microns/second, classified sperm with an efficiency of 98%, whereas the rule for the CellTrak system, WOB < or = 0.6 and VCL > or = 55 microns/second, achieved a classification efficiency of 97%. These rules should facilitate the study of sperm hyperactivation and its role in sperm function.


This article has been cited by other articles:


Home page
 J AndrolHome page
J. Baumber and S. A. Meyers
Hyperactivated Motility in Rhesus Macaque (Macaca mulatta) Spermatozoa
J Androl, May 1, 2006; 27(3): 459 - 468.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
J. Baumber and S. A. Meyers
Hyperactivated Motility in Rhesus Macaque (Macaca mulatta) Spermatozoa
J Androl, May 1, 2006; 27(3): 459 - 468.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
P. Caballero-Campo, M. Chirinos, X. J. Fan, M. E. Gonzalez-Gonzalez, M. Galicia-Chavarria, F. Larrea, and G. L. Gerton
Biological Effects of Recombinant Human Zona Pellucida Proteins on Sperm Function
Biol Reprod, April 1, 2006; 74(4): 760 - 768.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by The American Society of Andrology.