Journal of Andrology Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Melendrez, C. S.
Right arrow Articles by Casillas, E. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Melendrez, C. S.
Right arrow Articles by Casillas, E. R.

Journal of Andrology, Vol 13, Issue 4 293-296, Copyright © 1992 by The American Society of Andrology

JOURNAL ARTICLE

Polyamines in ejaculated ram spermatozoa and their relationship with sperm motility

C. S. Melendrez, J. L. Ruttle, D. M. Hallford, P. S. Chaudhry and E. R. Casillas
Department of Chemistry, New Mexico State University, Las Cruces 88003.

Intracellular polyamine levels in ejaculated spermatozoa and seminal fluid from rams were determined by fluorescent spectroscopy of their dansyl derivatives. Relationships between the sperm polyamine content and sperm motility of six mature and eight pubescent rams were studied. Samples were collected from both groups once a month from August through October. Mature rams had a greater percentage of motile sperm cells than lambs (94% versus 73% in September and 92% versus 78% in October); higher spermidine content (36 versus 9 pmol/10(8) cells in September and 162 versus 55 pmol/10(8) cells in October); higher spermine content (984 versus 205 pmol/10(8) cells in September and 1,229 versus 414 pmol/10(8) cells in October); and higher total sperm polyamine content (1,021 versus 216 pmol/10(8) cells in September and 2,258 versus 973 pmol/10(8) cells in October). In the lambs, spermidine content increased (55 versus 9 pmol/10(8) cells); spermine content increased (414 versus 205 pmol/10(8) cells); and total sperm polyamine content increased (973 versus 215 pmol/10(8) cells) in October compared to September. Ejaculates with sperm motility higher than 85% had greater spermine (848 versus 234 pmol/10(8) cells in September and 1064 versus 449 pmol/10(8) cells in October), and total sperm polyamine content (882 versus 244 pmol/10(8) cells in September and 2,015 versus 1,008 pmol/10(8) cells in October) than ejaculates with less than 450 pmol total sperm polyamines/10(8) cells was 68% +/- 6% compared to 90% +/- 4% in cells with greater than 450 pmol (average for all ejaculates) total sperm polyamines/10(8) cells. These data suggest a positive relationship between sperm polyamine constant and sperm motility.(ABSTRACT TRUNCATED AT 250 WORDS)




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1992 by The American Society of Andrology.