Journal of Andrology, Vol 11, Issue 4 336-343, Copyright © 1990 by The American Society of Andrology

JOURNAL ARTICLE

Cellular regulation of follicle-stimulating hormone (FSH) binding in rat seminiferous tubules

M. Kangasniemi, A. Kaipia, J. Toppari, A. Perheentupa, I. Huhtaniemi and M. Parvinen
Department of Anatomy, University of Turku, Finland.

Stage-specific binding of follicle-stimulating hormone (FSH) was measured in rat seminiferous tubules. The binding in single-point assays was over 3-fold higher (P less than 0.05) in stages XIII to I than in stages VI to VII of the epithelial cycle. No difference was found between the equilibrium association constants (Ka) of FSH binding in stages XIV to IV (10 +/- 1.9 X 10(9) 1/mol) and VII to VIII (9.2 +/- 0.6 X 10(9) 1/mol, mean +/- SEM, n = 5). In another experiment, the testes were dosed locally with 3 Gy of 4 MV x-irradiation to selectively lower the number of spermatogonia. After irradiation, FSH binding in staged seminiferous tubule segments was measured when the desired types of spermatogenic cells were reduced in number. Seven days after irradiation when differentiating spermatogonia and preleptotene spermatocytes were reduced in number, FSH binding was decreased in all stages of the cycle, but the cyclic variation remained. Seventeen days after irradiation when intermediate and type B spermatogonia and spermatocytes up to diplotene of stage XIII showed low numbers, FSH binding was decreased in all stages of the cycle and the stage-dependent variation disappeared. At 38 days when pachytene spermatocytes and early spermatids were reduced in number, similar results were found. But at 52 days postirradiation when all spermatids were low in number, FSH binding was slightly elevated compared with days 17 and 38. There were no significant differences in serum FSH or LH levels between irradiated and non-irradiated animals. These findings suggest that all spermatogenic cell types may stimulate FSH binding in the Sertoli cells.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				T. Ohta, H. Miyake, C. Miura, H. Kamei, K. Aida, and T. Miura Follicle-Stimulating Hormone Induces Spermatogenesis Mediated by Androgen Production in Japanese Eel, Anguilla japonica Biol Reprod, December 1, 2007; 77(6): 970 - 977. [Abstract] [Full Text] [PDF]

				M. Yamamoto, A. Narita, M. Kagohata, M. Shirai, F. Akahori, and K. Arishima Effects of Maternal Exposure to 3,3',4,4',5-Pentachlorobiphenyl (PCB126) or 3,3',4,4',5,5'-Hexachlorobiphenyl (PCB169) on Testicular Steroidogenesis and Spermatogenesis in Male Offspring Rats J Androl, March 1, 2005; 26(2): 205 - 214. [Abstract] [Full Text] [PDF]

				C. Foresta, A. Bettella, D. Spolaore, M. Merico, M. Rossato, and A. Ferlin Suppression of the high endogenous levels of plasma FSH in infertile men are associated with improved Sertoli cell function as reflected by elevated levels of plasma inhibin B Hum. Reprod., June 1, 2004; 19(6): 1431 - 1437. [Abstract] [Full Text] [PDF]

				M. J. Scobey, C. A. Fix, and W. H. Walker The Id2 Transcriptional Repressor Is Induced by Follicle-stimulating Hormone and cAMP J. Biol. Chem., April 16, 2004; 279(16): 16064 - 16070. [Abstract] [Full Text] [PDF]

				S. N. Kalantaridou and G. P. Chrousos Monogenic Disorders of Puberty J. Clin. Endocrinol. Metab., June 1, 2002; 87(6): 2481 - 2494. [Full Text] [PDF]

				W. Xing and M. R. Sairam Role of CACC-Box in the Regulation of Ovine Follicle-Stimulating Hormone Receptor Expression Biol Reprod, October 1, 2001; 65(4): 1142 - 1149. [Abstract] [Full Text] [PDF]

				L. L. Heckert Activation of the Rat Follicle-Stimulating Hormone Receptor Promoter by Steroidogenic Factor 1 Is Blocked by Protein Kinase A and Requires Upstream Stimulatory Factor Binding to a Proximal E Box Element Mol. Endocrinol., May 1, 2001; 15(5): 704 - 715. [Abstract] [Full Text]

				L. M. Grønning, J. E. Wang, A. H. Ree, T. B. Haugen, K. Taskén, and K. A. Taskén Regulation of Tissue Inhibitor of Metalloproteinases-1 in Rat Sertoli Cells: Induction by Germ Cell Residual Bodies, Interleukin-1{alpha}, and Second Messengers Biol Reprod, April 1, 2000; 62(4): 1040 - 1046. [Abstract] [Full Text]

				C. K. Jonsson, R. H. Zetterström, M. Holst, M. Parvinen, and O. Söder Constitutive Expression of Interleukin-1{alpha} Messenger Ribonucleic Acid in Rat Sertoli Cells Is Dependent upon Interaction with Germ Cells Endocrinology, August 1, 1999; 140(8): 3755 - 3761. [Abstract] [Full Text]

				A. Dierich, M. R. Sairam, L. Monaco, G. M. Fimia, A. Gansmuller, M. LeMeur, and P. Sassone-Corsi Impairing follicle-stimulating hormone (FSH) signaling in vivo: Targeted disruption of the FSH receptor leads to aberrant gametogenesis and hormonal imbalance PNAS, November 10, 1998; 95(23): 13612 - 13617. [Abstract] [Full Text] [PDF]

				M. Simoni, J. Gromoll, and E. Nieschlag The Follicle-Stimulating Hormone Receptor: Biochemistry, Molecular Biology, Physiology, and Pathophysiology Endocr. Rev., December 1, 1997; 18(6): 739 - 773. [Abstract] [Full Text]