Journal of Andrology Cross-Journal Searching
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Skinner, T. J.
Right arrow Articles by Heindel, J. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Skinner, T. J.
Right arrow Articles by Heindel, J. J.

Journal of Andrology, Vol 11, Issue 3 293-300, Copyright © 1990 by The American Society of Andrology

JOURNAL ARTICLE

Identification and characterization of a beta-adrenergic receptor in hamster Sertoli cells

T. J. Skinner and J. J. Heindel
Department of Biology, University of Mississippi.

Sertoli cells cultured from immature hamsters contain a beta-adrenergic receptor which is coupled to the cAMP second messenger system. Thus, isoproterenol, epinephrine, and norepinephrine, which act via beta-adrenergic receptors, all stimulate cAMP accumulation in Sertoli cells cultured for 4-5 days. This cAMP response to isoproterenol is inhibited stereospecifically by the beta-receptor blocker, propranolol. It is also sensitive to inhibition by beta-adrenergic antagonists in this order of potency: nonspecific beta receptor antagonists, propranolol, timolol, hydroxypindolol greater than beta 1 selective antagonists, oxyprenolol, metoprolol much much greater than beta 2 selective antagonist, butoxamine. Butoxamine was at least 1000-fold less sensitive than either the nonspecific or the beta 1 selective antagonists at inhibiting the response of either isoproterenol (nonspecific), dobutamine (beta 1 selective) or zinterol (beta 2 selective). The hamster Sertoli cell beta receptor is, therefore, predominantly of the B1 subtype. This beta receptor mediated increase in cAMP is sensitive to homologous desensitization and is stimulated synergistically by forskolin. In addition, Seroli cells freshly isolated from immature hamsters contain an active beta receptor. However, this beta receptor mediated increase in cAMP is dependent on the type of trypsin used in the cell preparation. In agreement with Kierszenbaum et al (1985), freshly isolated Sertoli cells from immature rats never responded to the catecholamines regardless of the type of trypsin used; indicating an important physiologic difference between rat and hamster Sertoli cells.


This article has been cited by other articles:


Home page
 EndocrinologyHome page
M. O. R. Borges, M. L. C. Abreu, C. S. Porto, and M. C. W. Avellar
Characterization of Muscarinic Acetylcholine Receptor in Rat Sertoli Cells
Endocrinology, November 1, 2001; 142(11): 4701 - 4710.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1990 by The American Society of Andrology.