Journal of Andrology Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Focarelli, R.
Right arrow Articles by Terrana, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Focarelli, R.
Right arrow Articles by Terrana, B.

Journal of Andrology, Vol 11, Issue 2 97-104, Copyright © 1990 by The American Society of Andrology

JOURNAL ARTICLE

Sialyglycoconjugates release during in vitro capacitation of human spermatozoa

R. Focarelli, F. Rosati and B. Terrana
Department of Evolutionary Biology, Siena, Italy.

Modifications of the human sperm surface during incubation in capacitating conditions were studied by radiolabeling terminal sialic acid residues of cell surface glycoconjugates using the sodium metaperiodate/sodium tritiated borohydride method. During in vitro capacitation, sialyglycoconjugates were released from the human sperm surface according to well reproducible kinetics. This release could be inhibited by the presence of seminal plasma in the capacitation buffer. Two principal size classes of sialyglycoconjugates were detected in the capacitation medium and analyzed by gel filtration chromatography and SDS-PAGE. The smaller class was characterized by glycopeptides less than 5,000 Da, whereas the larger class was characterized by two sialyglycoproteins of approximately 15,000-16,000 and 22,000-23,000 Mr. The role of human albumin, a key component of the capacitation buffer, in the removal of these molecules from the sperm surface was studied in light of its constant association with large amounts of released material.


This article has been cited by other articles:


Home page
 Hum ReprodHome page
T. L. Tollner, A. I. Yudin, C. A. Treece, J. W. Overstreet, and G. N. Cherr
Macaque sperm coating protein DEFB126 facilitates sperm penetration of cervical mucus
Hum. Reprod., November 1, 2008; 23(11): 2523 - 2534.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
N.C. Feki, P. Therond, M. Couturier, G. Limea, A. Legrand, P. Jouannet, and J. Auger
Human sperm lipid content is modified after migration into human cervical mucus
Mol. Hum. Reprod., February 1, 2004; 10(2): 137 - 142.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. I. Yudin, T. L. Tollner, M.-W. Li, C. A. Treece, J. W. Overstreet, and G. N. Cherr
ESP13.2, a Member of the {beta}-Defensin Family, Is a Macaque Sperm Surface-Coating Protein Involved in the Capacitation Process
Biol Reprod, October 1, 2003; 69(4): 1118 - 1128.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
R. Focarelli, S. Francavilla, F. Francavilla, C. D. Giovampaola, A. Santucci, and F. Rosati
A sialoglycoprotein, gp20, of the human capacitated sperm surface is a homologue of the leukocyte CD52 antigen: analysis of the effect of anti-CD52 monoclonal antibody (CAMPATH-1) on capacitated spermatozoa
Mol. Hum. Reprod., January 1, 1999; 5(1): 46 - 51.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
B. Gadella, T. Gadella, B Colenbrander, L. van Golde, and M Lopes-Cardozo
Visualization and quantification of glycolipid polarity dynamics in the plasma membrane of the mammalian spermatozoon
J. Cell Sci., January 8, 1994; 107(8): 2151 - 2163.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1990 by The American Society of Andrology.