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Journal of Andrology, Vol 1, Issue 3 138-144, Copyright © 1980 by The American Society of Andrology

Estradiol-induced Inhibition of Testosterone Production by Testicular Interstitial Cells Incubated In Vitro

H. E. GROTJAN JR. 1, L. J. RODRIGUEZ-RIGAU 1, AND E. STEINBERGER 1

1 Department of Reproductive Medicine and Biology, The University of Texas Medical School, Houston, Texas

Testosterone production by testicular interstitial cells incubated in vitro in the presence of 10-8 to 10-6 M 17beta-estradiol (E2), 17 beta-estradiol-3-benzoate (E2B), or benzoic acid (B) was examined. High concentrations of E2 (10-6 M), but not E2B or B, inhibited hCG-induced testosterone production (sim20%), but only if E2 and hCG were added at the beginning of the incubation. Preincubation (2-6 hours) of interstitial cells with 10-6 to 10-8 M E2 did not alter hCG-induced steroidogenesis. The inhibitory action of E2 was characterized as a decrease in the testosterone production rate and a decrease in the response to a maximal dose of hCG but did not involve a shift in the hCG dose response curve. Under the conditions employed, interstitial cells did not metabolize E2. These results are consistent with the view that estrogens do not have a direct inhibitory effect on interstitial cell steroidogenesis during the first few hours after systemic injection.

     Key words: testosterone, interstitial cells, estradiol, testis, steroidogenesis

Submitted on August 28, 1979
Revised on November 9, 1979
Accepted on November 16, 1979






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Copyright © 1980 by The American Society of Andrology.